Overview: Engineering Transgenic Constructs and Mice

An Overview of Challenges in Producing and Consuming Transgenic Products

The production and consumption of genetically modified (GM) products are highly controversial due to their environmental, health, and ethical impacts. Most of these disputes are caused by distrust of regulatory authorities, scientists, and technocratic decisions. Among all these concerns, health issues, allergenicity and antibiotic resistance are more important. Many of today's social developme...

Major approaches for generating and analyzing transgenic mice. An overview.

Over the past decade, the development of gene-transfer technology in whole animals has afforded unprecedented opportunities for investigators to probe complex regulatory systems in vivo. Important advances in our understanding of the mechanisms of gene expression and regulation and the development of animal models of human diseases are but two examples of how this technology has affected medica...

Overview on Vascular Tissue Engineering: Progress and Challenges

Today, vascular diseases such as atherosclerosis are one of the leading causes of death in the world and the prevalence of it in older societies is rising. The current treatments for repair of cardiovascular function include organ transplantation, surgical reconstruction, mechanical or artificial devices, or the use of metabolic products. Although these methods are commonly used, they did not g...

An Overview of Constructs and Selected Measures

Globin gene switching in transgenic mice carrying HS2-globin gene constructs.

We have examined the pattern of human globin gene switching in transgenic mice containing three different gamma and beta gene constructs (HS2G gamma A gamma delta beta, HS2A gamma beta neo, and HS2A gamma en beta) and compared the results with previously described transgenics (HS2A gamma beta, HS2G gamma A gamma-117 delta beta, and LCR epsilon G gamma A gamma delta beta). Developmental regulati...